Choose program to use and database to search: Program blastn blastp blastx tblastn tblastx Database KS01 KS07 KS08 KS09 KS10 KS11 KS12 KS13 KS14 KS15 KS16 KS17 KS18 KS19 KS20 KS21 KS22 KS23 KS24 KS25 KS26 LE.cons LE.seq PEPPER_CONS PEPPER_TOT SGN_TOT_EST Enter sequence below in FASTA format Or load it from disk Set subsequence: From         To

The query sequenc e is filtered for low complexity regions by default. Filter Low complexity Mask for lookup table only Expect 0.0001 0.01 1 10 100 1000    Matrix PAM30 PAM70 BLOSUM80 BLOSUM62 BLOSUM45 Perform ungapped alignment Query Genetic Codes (blastx only) Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial; ... (4) Invertebrate Mitochondrial (5) Ciliate Nuclear; ... (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15) Database Genetic Codes (tblast[nx] only) Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial; ... (4) Invertebrate Mitochondrial (5) Ciliate Nuclear; ... (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15) Frame shift penalty for blastx 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 25 30 50 1000 No OOF Other advanced options:

Graphical Overview Alignment view Pairwise master-slave with identities master-slave without identities flat master-slave with identities flat master-slave without identities BLAST XML Hit Table Descriptions 0 10 50 100 250 500    Alignments 0 10 50 100 250 500 Color schema No color schema Color schema 1 Color schema 2 Color schema 3 Color schema 4 Color schema 5 Color schema 6